Application

Research CategoryNeuroscience

Western blot: 1:1,000 using ECL. The antibody reacts with proteins of ~120 kDa. Preferred gel percentage is 7.5%. Suggested blocking buffer is PBS containing 5% non-fat milk. Suggested dilution buffer is PBS containing 1% non-fat milk.

Immunoprecipitation: 5 µL to 1 mL of cell extract. Preferred tissue/cell lysis buffer is RIPA. Suggested incubation time is 2-4 hours. Suggested capture agent is Protein A.

Optimal working dilutions must be determined by the end user.

Research Sub CategoryNeurochemistry & Neurotrophins

Anti-Cysteine-rich Motor Neuron 1 Antibody detects level of Cysteine-rich Motor Neuron 1 & has been published & validated for use in IP & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Recombinant protein from the cytoplasmic tail of mouse Crim1.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Rabbit serum. Liquid.

Specificity

Crim1

SUBCELLULAR LOCALIZATION: Intracellular and Membrane associated.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.